Project Name: Iron Uptake Systems listed on the Drug Discovery page.
A front line defense mechanism is sequestering iron to limit growth of pathogenic organisms. My research is aimed at understanding the mechanisms used by bacterial pathogens to acquire growth essential iron from human host sources. These iron uptake systems are required for full virulence of the pathogen and many components are specific receptors found at the cell surface. The surface receptors are proven targets for vaccine development and many components have the potential to be exploited therapeutically.
Relevant NGDI Articles
Improved manganese-oxidizing activity of DypB, a peroxidase from a lignolytic bacterium. Singh R, Grigg JC, Qin W, Kadla JF, Murphy ME, Eltis LD. ACS Chem Biol. 2013 Apr 19;8(4):700-6.
Mechanism of ferrous iron binding and oxidation by ferritin from a pennate diatom. Pfaffen S, Abdulqadir R, Le Brun NE, Murphy ME. J Biol Chem. 2013 May 24;288(21):14917-25.
Role of Rhodobacter sphaeroides photosynthetic reaction center residue M214 in the composition, absorbance properties, and conformations of H(A) and B(A) cofactors. Saer RG, Hardjasa A, Rosell FI, Mauk AG, Murphy ME, Beatty JT. Biochemistry. 2013 Apr 2;52(13):2206-17.
The Mannoprotein Cig1 supports iron acquisition from heme and virulence in the pathogenic fungus Cryptococcus neoformans. Cadieux B, Lian T, Hu G, Wang J, Biondo C, Teti G, Liu V, Murphy ME, Creagh AL, Kronstad JW. J Infect Dis. 2013 Apr 15;207(8):1339-47.
Discovery of an iron-regulated citrate synthase in staphylococcus aureus. Cheung J, Murphy MEP and Heinrichs DE. Chem Biol 2012 19(12):1568-1578.