Canada Research Chair in Pathogenomics and Antimicrobials
Microbiology & Immunology | Faculty of Science
Director | Centre for Microbial Diseases & Immunity Research
Co-Founder | Centre for Drug Research & Development
Co-Founder | Neglected Global Diseases Initiative
Research Interests:

Project Names: Innate Defence Regulators and Vaccine Adjuvants listed on the Drug Development page, and InnateDB listed on the Vector control, Tools & Diagnostics page.

My research interests include antibiotic uptake and resistance, the functional genomics of innate immunity and of the prominent opportunistic pathogen Pseudomonas aeruginosa, and the development of small cationic peptides as novel antimicrobials, adjuvant components and modulators of innate immunity. I have considerable experience with systems biology approaches to understanding the immune system.

Relevant NGDI Articles


Ability of innate defence regulator peptides IDR-1002, IDR-HH2 and IDR-1018 to protect against Mycobacterium tuberculosis infections in animal models. Rivas-Santiago B, Castañeda-Delgado JE, Rivas Santiago CE, Waldbrook M, González-Curiel I, León-Contreras JC, Enciso-Moreno JA, del Villar V, Mendez-Ramos J, Hancock RE, Hernandez-Pando R. PLoS One. 2013;8(3):e59119.

Armand-Frappier outstanding student award — role of ATP-dependent proteases in antibiotic resistance and virulence. Breidenstein EB,  Hancock RE. Can J Microbiol. 2013 Jan;59(1):1-8. Review.

Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies. de la Fuente-Núñez C, Reffuveille F, Fernández L, Hancock RE. Curr Opin Microbiol. 2013 Oct;16(5):580-9.

Characterization of a proteolytically stable multifunctional host defense peptidomimetic. Jahnsen RD, Haney EF, Franzyk H, Hancock RE. Chem Biol. 2013 Oct 24;20(10):1286-95.

Characterization of the polymyxin B resistome of Pseudomonas aeruginosa. Fernández L, Alvarez-Ortega C, Wiegand I, Olivares J,  Kocíncová D, Lam JS, Martínez JL, Hancock RE. Antimicrob Agents Chemother. 2013 Jan;57(1):110-9.

Effect of nitroxides on swarming motility and biofilm formation, multicellular behaviors in Pseudomonas aeruginosa. de la Fuente-Núñez C, Reffuveille F, Fairfull-Smith KE, Hancock RE. Antimicrob Agents Chemother. 2013 Oct;57(10):4877-81.

Expression of an engineered heterologous antimicrobial peptide in potato alters plant development and mitigates normal abiotic and biotic responses. Goyal RK, Hancock RE, Mattoo AK, Misra S. PLoS One. 2013 Oct 16;8(10):e77505. eCollection 2013.

Genome-wide identification of genes conferring energy related resistance to a synthetic antimicrobial peptide (Bac8c). Spindler EC, Boyle NR, Hancock RE, Gill RT. PLoS One. 2013;8(1):e55052.

Immune modulation by multifaceted cationic host defense (antimicrobial) peptides. Hilchie AL, Wuerth K, Hancock RE. Nat Chem Biol. 2013 Dec;9(12):761-8. Review.

Impaired pulmonary defense against Pseudomonas aeruginosa in VEGF gene inactivated mouse lung. Breen EC, Malloy JL, Tang K, Xia F, Fu Z, Hancock RE, Overhage J, Wagner PD, Spragg RG. J Cell Physiol. 2013 Feb;228(2):371-9.

Innate defence regulator peptide 1018 protects against perinatal brain injury. Bolouri H, Sävman K, Wang W, Thomas A, Maurer N, Dullaghan E, Fjell CD, Ek CJ, Hagberg H, Hancock RE, Brown KL, Mallard C. Ann Neurol. 2013 Dec 13.

INVEX–a web-based tool for integrative visualization of expression data. Xia J, Lyle NH, Mayer ML, Pena OM, Hancock RE. Bioinformatics. 2013 Dec 15;29(24):3232-4.

Multilayered coating on titanium for controlled release of antimicrobial peptides for the prevention of implant-associated    infections. Kazemzadeh-Narbat M, Lai BF, Ding C, Kizhakkedathu JN, Hancock RE, Wang R. Biomaterials. 2013 Aug;34(24):5969-77.

New strategies and compounds for anti-infective treatment. Hancock RE, Sahl HG. Curr Opin Microbiol. 2013 Oct;16(5):519-21.

Novel vaccine formulations against pertussis offer earlier onset of immunity and provide protection in the presence of maternal antibodies. Polewicz M, Gracia A, Garlapati S, van Kessel J, Strom S, Halperin SA, Hancock RE, Potter AA, Babiuk LA, Gerdts V. Vaccine. 2013 Jun 28;31(31):3148-55.

Peptide design for antimicrobial and immunomodulatory applications. Haney EF, Hancock RE. Biopolymers. 2013 Nov;100(6):572-83.  Link

Pseudomonas aeruginosa: new insights into pathogenesis and host defenses. Gellatly SL, Hancock RE. Pathog Dis. 2013 Apr;67(3):159-73. Review.

Requirement of the Pseudomonas aeruginosa CbrA Sensor Kinase for Full Virulence in a Murine Acute Lung Infection Model. Yeung AT, Janot L, Pena OM, Neidig A, Kukavica-Ibrulj I, Hilchie A, Levesque RC, Overhage J, Hancock RE. Infect Immun. 2014 Mar;82(3):1256-67. Epub 2013 Dec 30.

Synthetic analogues of bovine bactenecin dodecapeptide reduce  herpes simplex virus type 2 infectivity in mice. Shestakov A, Jenssen H, Hancock RE, Nordström I, Eriksson K. Antiviral Res. 2013 Nov;100(2):455-9.

The extra-cytoplasmic function sigma factor sigX modulates biofilm and virulence-related properties in Pseudomonas aeruginosa. Gicquel G, Bouffartigues E, Bains M, Oxaran V, Rosay T, Lesouhaitier O, Connil N, Bazire A, Maillot O, Bénard M, Cornelis P, Hancock RE, Dufour A, Feuilloley MG, Orange N, Déziel E, Chevalier S. PLoS One. 2013 Nov 18;8(11):e80407. eCollection 2013.

The innate defense regulator peptides IDR-HH2, IDR-1002, and IDR-1018 modulate human neutrophil functions. Niyonsaba F, Madera L, Afacan N, Okumura K, Ogawa H, Hancock RE. J Leukoc Biol. 2013 Jul;94(1):159-70.


A systems biology approach to nutritional immunology-focus on innate immunity. Afacan NJ, Fjell CD and Hancock REW. Molec Aspects Medicine 2012 33:14-25.

Adaptive and mutational resistance: Role of porins and efflux pumps in drug resistance. Fernández L and Hancock REW. Clin Microbiol Rev 2012 25(4):661-681.

Biomembrane interactions reveal the mechanism of action of surface-immobilized host defense IDR-1010 peptide. Gao G, Cheng JTJ, Kindrachuk J, Hancock REW, Straus SK and Kizhakkedathu JN. Chem Biol 2012 19(2):199–209.

Comparison of immune responses and protective efficacy of intranasal prime-boost immunization regimens using adenovirus-based and CpG/HH2 adjuvanted-subunit vaccines against genital Brown THT, David J, Acosta-Ramirez E, Moore1 JM, Lee S, Zhong G, Hancock REW, Xing Z, Halperin SA and Wang J. Chlamydia muridarum infection. Vaccine 2012 30(2):350-360.

Designing antimicrobial peptides: Form follows function. Fjell CD, Hiss JA, Hancock REW, and Schneider G. Nat Rev Drug Discov 2012 11:37-51.

Drug release and bone growth studies of antimicrobial peptide-loaded calcium phosphate coating on titanium. Kazemzadeh-Narbat M, Noordin S, Masri BA, Garbuz DS, Duncan CP,  Hancock REW and Wang R. J Biomed Mater Res B-Appl Biomater 2012 100(5):1344-52.

Enhanced immune responses and protection by vaccination with respiratory syncytial virus fusion protein formulated with CpG oligodeoxynucleotide and synthetic innate defense regulator peptide in polyphosphazene microparticles. Garlapati S, Garg R, Brownlie R, Latimer L, Simko E, Hancock REW, Babiuk L, Gerdts V, Potter A and van den Hurk S. Vaccine 2012 30(35):5206-14.

Inhibition of bacterial biofilm formation and swarming motility by a small synthetic cationic peptide. de la Fuente-Núñez C, Korolik V, Bains M, Nguyen U, Breidenstein EBM, Horsman S, Lewenza S, Burrows L and Hancock REW. Antimicrob Agents Chemother 2012 56(5):2696-2704.

Innate defense regulator peptide 1018 in wound healing and wound infection. Steinstraesser L, Hirsch T, Schulte M, Kueckelhaus M, Jacobsen F, Mersch EA, Stricker I, Afacan N, Jenssen H, Hancock REW and Kindrachuk J. PLoS ONE 2012    7(8):e39373 epub.

Involvement of the Lon protease in the SOS response triggered by ciprofloxacin in Pseudomonas aeruginosa PAO1. Breidenstein EBM, Bains M and Hancock REW. Antimicrob Agents Chemother 2012 56(6):2879-2887.

Local delivery of antimicrobial peptides using self-organized TiO2nanotube arrays for peri-implant infections. Ma M, Kazemzadeh-Narbat M, Hui Y, Lu S, Ding C, Chen DDY, Hancock REW and Wang R. J Biomed Materials Res Part A 2012 100A(2):278-285.

LpxC inhibition protects mice from resistant Acinetobacter baumannii by modulating inflammation and enhancing phagocytosis. Lin L, Tan B, Pantapalangkoor P, Ho T, Baquir B, Tomaras A, Montgomery JI, Reilly U, Barbacci EG, Hujer K, Bonomo RA, Fernandez L, Hancock REW, Adams MD, French SW, Buslon VS and Spellberg B.mBio 2012 3(5) pii:e00312-12 epub.

Modulating immunity as a therapy for bacterial infections. Hancock REW, Nijnik A and Philpott DJ. Nature Rev Microbiol 2012 10(4):243-254.

Phosphate starvation promotes swarming motility and cytotoxicity in Pseudomonas aeruginosa. Bains M, Fernández L and Hancock REW. Appl Environ Microbiol 2012 78(18):6762-6768.

Protein Interaction Data Curation – The International Molecular Exchange Consortium (IMEx). Orchard S, Kerrien S, Abbani S, Aranda B, Bhate J, Bidwell S, Bridge A, Briganti L, Brinkman F, Cesareni G, Chatr-aryamontri A, Chautard E, Chen C, Dumousseau M, Eisenberg D, Goll J, Hancock REW, Hannick L, Jurisica I, Khadake J, Lynn DJ, Mahadevan U, Perfetto L, Raghunath A, Ricard-Blum S, Roechert B, Salwinski L, Stümpflen V, Tyers M, Uetz P, Xenarios I and Hermjakob H. Nature Methods 2012 9(4):345-350.

Role of intracellular proteases in the antibiotic resistance, motility, and biofilm formation of Pseudomonas aeruginosa. Fernández L, Breidenstein EBM, Song D and Hancock REW. Antimicrob Agents Chemother 2012 56(2):1128-1132.

Structure, dynamics, antimicrobial and immune modulatory activities of human LL-23 and its single residue variants mutated on the basis of homologous primate cathelicidins. Wang G, Elliott M, Cogen AL, Ezell EL, Gallo RL and Hancock REW. Biochemistry 2012 51(2):653-664.

Synthetic immunomodulatory peptide IDR-1002 enhances monocyte migration and adhesion on fibronectin. Madera L and Hancock REW. J Innate Immun 2012 4(5-6):553-568.

The bacterial surface layer provides protection against antimicrobial peptides. Fuente-Núñez C, Mertens J, Smit J and Hancock REW. Appl Environ Microbiol 2012 78(15):5452-5456. Covered in Nature Reviews Microbiology 10:442

The Pseudomonas aeruginosa phoP-phoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation. Gellatly SL, Needham B, Madera L, Trent MS and Hancock REW.  Infect Immun 2012 80(9):3122-3131.

The role of sphingosine-1-phosphate transporter Spns2 in immune system function. Nijnik A, Clare S, Hale C, Chen J, Raisen C, Mottram L, Lucas M, Estabel J, Ryder E, Adissu H, Sanger Mouse Genetics Project, Adams NC,  Ramirez-Solis R, White JK, Steel KP, Dougan G and Hancock REW. J Immunol 2012 189:102-111. Featured in the “In This Issue” section of The Journal of Immunology.

Therapeutic potential of host defense peptides in antibiotic-resistant infections. Afacan NJ, Yeung ATY, Pena OM and Hancock REW. Curr Pharm Design 2012 18(6):807-819.


Modulation of interleukin-1[beta]-induced inflammatory responses by a synthetic cationic innate defence regulator peptide, IDR-1002, in synovial fibroblasts. Turner-Brannen E, Choi K-Y, Lippert DND, Corten JP, Hancock REW, El-Gabalawy H, Arthritis Research & Therapy 2011, Vol13:R129.

The human cathelicidin, LL-37, induces granzyme-mediated apoptosis in cytotoxic T lymphocytes. Mader JS, Marcet-Palacios M, Hancock REW, and Bleackley RC.  Experimental Cell Research 2011, Vol317:4:531-538.

The human transcriptome during nontyphoid Salmonella and HIV coinfection reveals attenuated NFjB-mediated inflammation and persistent cell cycle disruption. Schreiber F, Lynn DJ, Houston A, Peters J, Mwafulirwa G, Finlay B, Brinkman FSL, Hancock REW, Heyderman RS, Dougan G, and Gordon MA.  The Journal of Infectious Diseases 2011, 204:1237-45.