Coming from a background in the investigation of enzyme mechanisms, protein structure/function relationships, enzyme inhibitor design/synthesis and protein engineering we are increasingly adopting and developing tools to carry out such studies in a high-throughput manner. An area of interest is in the discovery of enzyme inhibitors or chaperones as novel pharmaceuticals through high-throughput screening. Recent successes include potential treatments for diabetes and influenza. In each case we are targeting key carbohydrate synthesizing or degrading enzymes.
Relevant NGDI Articles
A plate-based high-throughput activity assay for polysialyltransferase from Neisseria meningitidis. Yu CC, Hill T, Kwan DH, Chen HM, Lin CC, Wakarchuk W, Withers SG. Anal Biochem. 2014 Jan 1;444:67-74.
Insights into mucopolysaccharidosis I from the structure and action of α-L-iduronidase. Bie H, Yin J, He X, Kermode AR, Goddard-Borger ED, Withers SG, James MN. Nat Chem Biol. 2013 Nov;9(11):739-45. Erratum in: Nat Chem Biol. 2013 Nov;9(11):746.
The structure of the Mycobacterium smegmatis trehalose synthase reveals an unusual active site configuration and acarbose-binding mode. Caner S, Nguyen N, Aguda A, Zhang R, Pan YT, Withers SG, Brayer GD. Glycobiology. 2013 Sep;23(9):1075-83.